Journal: The Journal of Neuroscience

Article Title: Limiting Neuronal Nogo Receptor 1 Signaling during Experimental Autoimmune Encephalomyelitis Preserves Axonal Transport and Abrogates Inflammatory Demyelination

doi: 10.1523/JNEUROSCI.1760-18.2019

Figure Lengend Snippet: Primary antibodies used in study

Article Snippet: Mouse anti-NgR 1:100 MAB1208 R&D Systems Human post-mortem MS brain immunofluorescence Rabbit anti-proteolipid (PLP) 1:500 Ab28486 Abcam Human post-mortem MS brain immunofluorescence Rabbit anti-pCRMP2-T555 1:40,000 ( Petratos et al., 2012 ; Mokhtar et al., 2018 ) Western blot Mouse anti-CRMP2 1:40,000 11096 IBL Western blot Rabbit anti-KIF5c 1:4000 LS- {"type":"entrez-nucleotide","attrs":{"text":"C81914","term_id":"33650884","term_text":"C81914"}} C81914 LSBio Western blot Mouse anti-β-actin 1:50,000 MAB1501 Millipore Western blot Mouse anti-Kinesin light chain-1 (KLC-1) 1:2000 MAB1617 Millipore Western blot Mouse anti-α-tubulin 1:10,000 05-829 Millipore Western blot Biotinylated anti-GFP 1:200 Ab6658 Abcam Oligodendrocyte apoptosis assay Mouse anti-CC1 1:50 {"type":"entrez-nucleotide","attrs":{"text":"M11217","term_id":"333925"}} M11217 Calbiochem Oligodendrocyte apoptosis assay Rabbit anti-cleaved caspase-3 1:400 9661 Cell Signaling Technology Oligodendrocyte apoptosis assay Open in a separate window Primary antibodies used in study table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Antibody Dilution Catalogue Supplier Application AlexaFluor 488 conjugated goat anti-rabbit IgG (H + L) 1:500 A-11008 Life Technologies Retina whole-mount, Optic nerve immunofluorescence AlexaFluor 546 conjugated goat anti-mouse IgG (H + L) 1:500 A-11030 Life Technologies Retina whole-mount, Optic nerve immunofluorescence, Oligodendrocyte apoptosis assay AlexaFluor 647 conjugated goat anti-rat IgG (H + L) 1:500 A-21247 Life Technologies Retina whole-mount, Optic nerve immunofluorescence, Oligodendrocyte apoptosis assay HRP-conjugated goat anti-mouse IgG (H + L) 1:20,000 AB308P Millipore Western blot HRP-conjugated goat anti-rabbit IgG (H + L) 1:20,000 AB307P Millipore Western blot Streptavidin, AlexaFluor 488 conjugated 1:500 {"type":"entrez-protein","attrs":{"text":"S11223","term_id":"112468","term_text":"pir||S11223"}} S11223 Life Technologies Oligodendrocyte apoptosis assay Open in a separate window Secondary antibodies used in study Quantitative PCR after reverse-transcription. mRNA was isolated from rAAV2-transduced retinae, and nontransduced retinae of naive ngr1 +/+ , ngr1 −/− , and ngr1 flx/flx mice 7 d post-intraocular injection by using RNeasy Mini kit (Qiagen) with DNase-I to remove genomic DNA.

Techniques: Immunofluorescence, Western Blot, Apoptosis Assay